Levels of total lipids in the seeds of three species of the Pinaceae family were determined and their fatty acid compositions were also analyzed by a gas-chromatograph equipped with a capillary column coated with Carbowax 20M. The results are summarized as follows: Lipid contents of the seeds amounted to 56.9% in P. koraiensis, 29.9% in P. thunbergii, and 21.2% in P. rigida. In all lipids 19${sim}$20 fatty acid were detected and, surprisingly, fatty acids having ${Delta}^5$-non-methylene interrupted conjugate double bond such as ${Delta}^{5, 9}-C_{18:2},{Delta}^{5, 9, 12}-C_{18:3};and;{Delta}^{5, 11, 14}-C_{20:3}$ occurred in appreciable amounts. In the lipids of P. koraiensis, the main component was $C_{18:2}{omega}_6(45.0%)$, followed by $C_18:1{omega} _9(26.9%)$ and ${Delta}^{5, 9, 12}-C_{18:3}(14.6%)$, and then ${Delta}^{5, 9}-C_{18:2}(2.2%)$ and ${Delta}^{5, 11, 14}-C_{20:3}$ were also present. Levels of saturated fatty acid such as $C_{16:0};and;C_{18:0}$ were as low as 7.5%. The seed oil of P. thunbergii predominantly comprised $C_{18:2}{omega}_6(45.2%)$, and was then occupied by equal amounts ${Delta}^{5, 9, 12}-C_{18:3}(18.1%)$ and $C_{18:1}{omega}_9(18.1%)$. Its ${Delta}^5, 11, 14}-C_{20:3}(5.8%)$ level was the highest in the samples tested. ${Delta}^{5, 9}-C_{18:2}(2.8%)$ was also detected with other minor components. In the oils from the seeds of P. rigida, $C_{18:2}{omega}_6$ was present as a main component, accompanied by $C_{18:1}{omega}_9(21.6%)$ and ${Delta}^{5, 9, 12}-C_{18:3}(20.3%)$. The latter showed higher level than in any other samples. A minor component corresponding to ${Delta}^{5, 9, 12, 15}-C_{18:4}$(not confirmed by GC-Mass) occurred in P. thunbergii and P. rigida.