We made biological investigation on the conservational environment of collections in the Ho Am museum. Annual average temperature and relative humidity outside the museum were $11. 0∼11.7^{circ}C$ and 64.8∼74.4% respectivey. On the other hand, average annual temperature and relative humidity inside the main storage were $19.1∼20.1^{circ}C$ and 53.0∼63.4%. We isolated fungi and classified into 8 genus 13species fungi and selected four fungi having high cellulotic activity such as Alternaria brassicae KCPRI 9202, Aspergillus niger KCPRI 9205, Aspergillusversicolor KCPRI 9206, Penicillium adametzi KCPRI 9208. These fungi were examined on the posibility of collections being damaged under current conservation al environment in the museum. KCPRI 9208 was non-tonophilic fungus and other were facutative tonophilic fungi. These showed maximal cellulotic activity of enzymeshaking culture at pH 5.0∼5.5 for 4 and 5 days. In proprtion to the period damaged, cellulase activity for paper damaged artifically with growing worse of material. As are sult cellulotic activity by fungi increased.