Pellicularia sasakii의 생육을 저해하는 한 균주를 토양으로부터 분리하였으며 Pseudomonas aeruginosa 3120으로 동정하였다. P. aeruginosa 3120의 배양액으로부터 분리 정제된 암갈색의 MRL3120 항생물질은 ethylacetate, chloroform 및 methanol에 용해되었으며 곰팡이뿐만 아니라 gram 양성 및 음성세균에도 활성을 나타내었다. UV, IR, EI-MS spectra 및 다른 물리화학적 특성의 분석결과로부터 MRL3120은 2개의 N-methyl-N-thioformylhydroxylamine과 1개의 Cu 이온으로 구성된 chelate 화합물로서 fluopsin C와 유사한 구조를 가진 것으로 추정되었다. Soybean meal을 포함하는 발효배지에 $CuSO_4$의 첨가는 항진균 활성을 증가시켰으나 EDTA(0.1%)의 존재하에서는 활성이 없었다. Soybean meal 대신 soytone을 포함하는 발효배지에서 MRL3120은 생산되지 않았지만 $CuSO_4$의 첨가에 의해 빠르게 생산되었다.
A strain that inhibited the growth of Pellicularia sasakii was isolated from the soil and identified as Pseudomonas aeruginosa 3120. A dark brownish antibiotic, MRL3120 isolated and purified from the culture broth of P. aeruginosa 3120 was soluble in ethylacetate, chloroform and methanol, and it was active against gram-positive and negative bacteria as well as fungi. The structure of MRL3120 was identified as a chelate compound consisting of two N-methyl-N-thioformyl-hydroxylamine and a copper ion by the analysis of UV, IR, and EI-MS spectra and other physico-chemical properties and supposed to have a structure of fluopsin C related compound. Addition of $CuSO_4$ into the fermentation medium containing soybean meal increased antifungal activity but no activity was found in the presence of EDTA (0.1%, v/v). However antibiotic MRL3120 was not produced in the fermentation medium containing soytone instead of soybean meal but it was rapidly produced by the addition of $CuSO_4$.
