- 인삼 산성다당체의 비색정량 방법과 그 추출조건 및 안정성
- ㆍ 저자명
- 도재호,이형옥
- ㆍ 간행물명
- 고려인삼학회지
- ㆍ 권/호정보
- 1993년|17권 2호|pp.139-144 (6 pages)
- ㆍ 발행정보
- 고려인삼학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
The method for colorimetric determination of acidic polysaccharide from Panax ginseng was investigated. It is possible to apply the method of carbazole-sulfuric acid to determination of pectin, and also to measure the amount of pectin in the mixture of various high molemlu compounds such as starch. cellulose and gum, etc. When the method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharide, optical density at 525 nm increased linearly with an increase in the concentration of pure acidic polysaccharide. Effective extraction temperature with water for the determination of the amount of ginseng acidic polysaccharide (GAP) was $80{circ}C$. In order to separate or concentrate GAP it was appropriate to precipitate the extract only once with 80% ethyl alcohol. GAP was very stable at $100{circ}C$ for 4 hrs in aqueous solution and between pH values of 5.0~ 12.0.