This study was investigated optimal conditions which were necessary to prepare emulsion of four component systems by the Microfluidizer. Squalane, isopropyl myristate, PPG-15 stearyl benzoate ester were used as an oil and POE(20) sorbitan monolaurate, POE(20) sorbitan monopalmitate, POE(20) sorbitan monostearate, glycerine were selected as a cosurfactant and surfactant. The emulsion was affected by the operating pressure, the recycle frequency and oil, surfactant, cosurfactant phase. The operating pressure and recycle frequency increased, the droplet diameter of emulsion decreased and was constant later. In this study, the optimal pressure and recycle frequency were 1400 bar, 4 cycles. The droplet size was the smallest when the concentration of glycerine was 30 wt.%. As the oil phase is increased, the size is increased.