Nitric oxide of gas phase in cigarette smoke was conveniently determined by Chemiluminescence NO analyzer with 20 ports rotary type smoking machine Data are presented to support this observation. On the basis of this method the amount of NO in the smoke from nonblended and commercial cigarettes were estimated. Gas phase NO concentration in main stream smoke was correlated well with the nitrate levels in tobacco leaves, but there was no correlation with the tar yields. In consequence, it would be considered that the methodology in this study provided simple and rapid quantification for NO in cigarette smoke.