This study was performed to investigate the effect of washing with water and detergent on the vitamin C content in strawberry and perilla leaf. Vitamin C was extracted with the solution of metaphosphoric acid and quantitated by high performance liquid chromatograph (HPLC). There was no significant difference of vitamin C content between the washing with distilled water and tap water. Three available detergents reduced the vitamin C content of samples to 92.5%, 92.2% and 78.5%, respectively. The content of vitamin C in samples was decreased by increasing the concentration of detergent and washing time.