The thesis describes the realization and test of the transport layer operating on the CLNS (Connection Less Network Service) which is specified by ISO 8073 and ADDENDUM 2 protocol. For realization of the transport layer, five modules and interface primitives were definde. This protocol was formally described by SDL (Specification and Description Language) and was programmed in C-language. For verification of the software, protocol tester was implemented and test scenarios were defined. The verification was accomplished based on the test scenarios. Transport Layer software presented in this thesis may be utilized in a real situation with some modifications. Protocol tester presented in this thesis can also be used for verification of other protocol softwares.