Most studies on the vibration analysis of a beam-column with ends elastically restrained and various intermediate constraints have been based on the Euler beam theory, which is inadequate for beam-columns of low slenderness ratios. In this paper, analytical methods for vibration and dynamic sensitivity of a Timoshenko beam-column with ends elastically restrained and various intermediate constraints are presented. Firstly, an exact solution method is shown. Since the exact method requires considerable computational effort, a Rayleigh-Ritz analysis is also investigated. In the latter two kinds of trial functions are examined for comparisions : eigenfunctions of the base system(the system without intermediate constraints) and polynomials having properties corresponding to the eigenfunctions of the base system. The results of some numerical Investigations show that the Rayleigh-Ritz analysis using the characteristic polynomials is competitive with the exact solutions in accuracy, and that it is much more efficient in computations than using the eigenfunctions of the base system, especially in the dynamic sensitivity analysis. In addition, the prediction of the changes of natural frequencies due to the changes of design variables based on the first order sensitivity is in good agreements with that by the ordinary reanalysis as long as the changes of design variables are moderate.