Some biological characteristics with respect to tuber formation of Eleocharis kuroguwai Ohwi were investigated. Tuber formation was initiated at the descending time of daily maximum and minimum temperatures, accelerated under the minimum temperature lower than $20^{circ}C$, and terminated at about $10^{circ}C$. Tubers produced at the early season were located at deeper soil layers. Large tubers were found at deeper soil depths and required the longer period for sprouting as compared with small tubers. Percent emergence decreased as the emergence depth increased, while percent old-tuber produced during previous years was greater at the deeper depths.