정제된 쥐의 간 시토졸 ALDH의 antiserum을 이용하여 Ouchterlony double diffusion 및 immunoblotting을 수행한 결과 쥐의 간 시토졸 ALDH는 매우 특이적이며 소의 간 시토졸 ALDH, 쥐의 간 미토콘드리아의 외막결합 ALDH와 간질분획에 존재하는 가용성 low $K_m$ ALDH와는 면역학적 상관관계가 없었고 활성 염색법으로 조사한 결과 antigenic determinant site가 활성부위가 아님을 알 수 있었다.
Specificity for cytosolic ALDH was investigated through ouchterlony double immunodiffusion and western blotting. The precipitin band formed by antigen-antibody was positive for protein staining as well as enzyme activity staining, indicating that an interaction of the antibody with antigenic determinants of the enzyme did not destroy a catalytic activity of this enzyme. It was realized that the immunological activity of rat liver cytosolic ALDH is quite specific and differs from those of other ALDH isozymes such as bovine liver cytosol ALDH and outer-membrane bound and matrix ALDH isozymes of rat liver. Results of activity staining of rat cytosol ALDH showed that antigenic determinant site is not active site.
