Chain drive power transmission system was developed for a low head hydro-turbine which generates power by energy transformation on the turbine blades attached to chains. Also, experimental and theoretical analysis for the sprocket wheel tooth load distribution were performed. The tooth load was measured by the specially designed load sensor. It was found that the tooth load distribution for the steady state operation was in good accordance with the quasi-static state results showing the peak load at the final meshing tooth. The trend of the experimental results agreed with the theoretical results based on the spring model analysis and difference in the magnitude of the maximum tooth load was considered to be the effect of the variable spring constant due to the moving contact point between the roller and sprocket wheel tooth.