In this study, Pullulan Production from whey medium by Aureohsidium pullulans was investigated. When the Pullulan production from whey medium was carried outs final cell concentration was similar to sucrose basal medium but Pullulan concentration was less than 5g/$ell$. For pullulan production from whey medium, adaptation culture technique was tried on lactose and galactose base medium When the adaptation culture technique was not applied, the maximum concentration of pullulan was 3.4g/$ell$ for lactose, 2.5g/$ell$ for galactose. Adapted strains produced 10~12g/$ell$ from lactose, 10~11g/$ell$ from galactose. The pullulan production from lactose basal medium was 13.5g/$ell$ for lactose adapted strains and 18.6g/$ell$ for galactose adapted strains. When the adapted strains were inoculated on whey medium, maximum pullulan production was obtained at initial pH 3.0.