In this thesis, the fatigue tests were performed on a series of reinforced concrete to Investigate the variation of strength and the safety of reinforced concrete structures under fatigue load. The specimens were of the same rectangular cross-section, of effective height 24cm and width 30cm and their span was 330cm. The three point loading system is used in the fatigue tests. In these tests, the fracture mode of reinforced concrete structures under fatigue load, relationship between the repeated loading cycles and the mid-span displacement of the specimens were observed. According to the test results, the following fatigue behavior of reinforced concrete specimens were observed. By increasing of the number of repeated loading cycles, the mid-span displacement became greater, however the Incremental amounts of the displacement were reduced. It could be also known that the inelastic strain energy of the doubly reinforced rectangular beams was larger than that of the singly reinforced rectangular beams as increasing the number of repeated loading cycles. Compliance of reinforced concrete structures tended to be reduced as increasing the repeated loading cycles, and the compliance of the doubly reinforced rectangular beams was generally smaller than that of the singly reinforced rectangular beams. Based on the above investigation, it could be concluded that the doubly reinforced rectangular beams under fatigue load were more efficient to resist the brittle fracture than the singly reinforced rectangular beams.