Morphology of glochidia of two species of Unionidae mussels, Unio douglasiae and Anodonta woodiana were studied based on SEM, and their attachment on Acheilognathus yamatsutae(Acheilognathinae) was observed in the experimentally infected fishes. The glochidia were round-triangle in shape and possessed larval threads, hooks, hooklets, and sensory hairs. Glochidal attachment and cyst formation of the fish were observed without regard to feeding habit and habitat. Density of glochida on the fish (Acheilognathus yamatsutae) was in the order of pectoral fin, dorsal fin, gill and caudal fin. The host fish became encysted about 5-10 hours to the glochidia, and the glochidia detached about 5-6 days after attachment at water temperature $26{pm}1^{circ}C$.