Fomitella fraxinea의 자실체로부터 열수 추출법에 의하여 다당류를 추출한 뒤, 분획정제하여 다당류에 대한 물리화학적 특성 및 항암 면역 활성을 조사하였다. 정제된 다당류에서 소량의 uronic acid가 측정되었으며, 주 구성당으로서 glucose, fucose, galactose, 및 mannose 가 존재하였고, 소량의 xylose, arabinose, 및 rhamnose가 검출되었다 . FHW-Ib에서 단백질은 측정되지 않았으며, FHW-Ia는 분자량이 19,000인 fuco-gluco-mannogalactan으로 FHW-Ib는 분자량 15,000인 gluco-fuco-mannogalactan으로 각각 동정되었다. FHW -IIa 및 FHW-IIb에서는 소량의 단백질이 측정되었고, 아미노산 분석 결과 threonine, glutamic acid, serine, alanine 및 aspartic acid의 양이 비교적 높게 존재하였다. FHW-IIa 및 FHW- IIb 는 galacto-mannogIucan 이며 분자량은 각각 31,000 및 9,000이었다. FHW-Ib 와 FHW-IIb 의 IR spectrum분석 결과 이 두 다당류는 ${eta}-type$이 아님이 밝혀졌다. 항암 면역 활성은 FHW-Ia, FHW-Ib, 및 FHW-IIa에서는 거의 없었고 FHW-IIb에서는 높았다.
Polysaccharide FHW was extracted from the fruiting bodies of Fomitella fraxinea with hot-water treatment and then fractionated into FHW-I and FHW-II on DEAE-Cellulose chromatography. FHW-I and FCW-II were further purified into FHW-Ia and Ib, FHW-IIa and IIb on gel permeation chromatography, respectively. A small amount of uronic acid was detected and glucose, galactose, fucose, and mannose were found to be main sugars in the polysaccharides. Protein was detected in FHW-Ia, FHW-IIa, and FHW-IIb, but not in FHW-Ib. FHW-Ia was identified to be a fuco-gluco-mannogalactan with molecular weight of 19,000 and FHW-Ib was a gluco-fuco-mannogalactan of 15,000. FHW-IIa and FHW-IIb were galacto-mannoglucan and their molecular weights were estimated to be 31,000 and 9,000, respectively. Both FHW-Ib and FHW-IIb did not show an absorption band characteristic of the ${eta}-glycosidic$ linkage in IR spectra. FHW-IIb showed a strong immuno-stimulating activity but the other three polysaccharides showed a weak activity.
