The study was conducted to investigate the effect of cultivation area with different sea levels on essential oil in the root of Valeriana fauriei var. dasycarpa Hara (Valerianaceae). The total content of the essential oil was not different in various cultivation area, however, its composition varied. The major components were bornyl acetate and sesquiterpene alcohol in three cultivation areas. Among the valepotrate components known as major active components in Europe and Nepal valerian, valtrate was detected in a small amount, but the quantity was not changed.