Opuntia dillenii Haw의 열매를 시료로 하여 betacyanins의 온도에 따른 안정성과 N-propyl gallate, L-cysteine, ascorbic acid에 대한 농도별 항산화효과를 조사하였다. Betacyanins의 최대 흡광도는 $536{sim}538;nm$에서 일어났으며 $90^{circ}C$에서 열처리 시간이 경과될수록 색소가 퇴색되면서 최대흡수파장이 자외선 영역으로 이동하는 현상을 나타냈다. Betacyanins는 열처리 온도에 대해 1차 반응 속도식에 따라 분해되었으며 $50^{circ}C$에서 $90^{circ}C$까지 열처리 했을때 반응 속도 상수가 $1.56{ imes}10^{-3}$분에서 $71.91{ imes}10^{-3}/min$분으로 증가하고 반감기가 444.23분에서 9.64분으로 감소하여 온도가 상승함에 따라 열안정성이 저하되는 결과를 보였다. 또한 betacyanins의 활성화 에너지는 10.94 kcal/mole로 나타났다. N-propyl gallate, L-cysteine, ascorbic acid를 $0.01{sim}0.3%$ 내로 첨가하여 $50^{circ}C,;70^{circ}C,;90^{circ}C$에서 betacyanins에 대한 항산화 효과를 비교한 결과 $70^{circ}C$와 $90^{circ}C$에서 미약한 항산화 효과를 보였으며 모든 온도에서 ascorbic acid는 $2{sim}10$배 정도의 반감기를 증가시켜 항산화 효과가 뛰어난 것으로 나타났다.
The color stability of betacyanins and effects of antioxidants from Opuntia dillenii Haw were determined in the fruit juice at temperature up to $90^{circ}C$. The absorption maxima of betacyanins occurred between 536 nm and 538 nm. When fruit juice was heated at $90^{circ}C$ for various times, the red color gradually diminished and the absorption maxima slightly shifted toward uv region. The kinetic analysis of the data obtained indicated that the discoloration for betacyanins obeyed first order reaction pattern, when the thermal stability test was performed at $50{sim}90^{circ}C$. And the rate constant increased from $1.56{ imes}10^{-3}/min;to;71.91{ imes}10^{-3}/min$ with the half-life decreasing from 444.23 min to 9.64 min. The results also indicated that the thermal stability of pigment decreased with increasing temperature. The energy of activation was 10.94 kcal/mole for the pigment. N-propyl gallate, L-cysteine, and ascorbic acid were added to cactus fruit juice at concentrations of $0.01{sim}0.3%$ at different temperatures. Npropyl gallate and L-cysteine had a little antioxidant effect on betacyanins stability at $50^{circ}C;and;70^{circ}C$, whereas ascorbic acid had a great antioxidant effect with the half-life value of 2 to 10 times to that of the control.
