This study was conducted to know the effect of soil moisture content on growth of aerial parts and root of Valeriana fauriei var. dayscarpa HARA in pot condition. The soil moisture contents were 30, 45, 55, 70, 80 and 90% of maximum water requirement. The result obtained are as follows; 1. Width and length of leaves in valerian were slightly increased with increase the soil moisture content, however, length of petiole and root were increased with soil moisture content up to 80%. 2. It showed the positive correlations between moisture content and root weight and extract content in root. 3. Optimum soil moisture content was 80 to 90% of maximum water requirement.