This paper deals with an experimental study on the dynamic behaviour of rubber oil seals when the interferences between the shaft and the seal lip as well as the dynamic eccentricities are present. The micro-separation of the sealing gap was observed with the aid of an image processing apparatus. The temperature of the seal lip edge, friction torque and the dynamic sealing gap profile are experimentally investigated for the initial interference and the shaft eccentricity. The data was simultaneously measured under the operation conditions. Experimental results show that, as the shaft speed is increased, the leakage of sealed fluids is increasing for a certain range of shaft speeds. The test data indicates that the shaft eccentricity clearly produces the gap separation between the shaft and the seal lip which is unable to follow the radial displacement of shaft as the shaft speed increases.