In this study, the effects of the power transmission units on the rotational accuracy are investigated experimentally in a hydrostatic spindle. The effects of warm up time, unbalancing and the position of measuring sensor are pre-examined for the determination of measuring conditions. The misalignment of the power transmission units and the vibration excited by the fluctuation of belt are considered as the dominant parameters of error motion. The variation and scatter of run out at the range of 0 to 3,000rpm in rotational speed are appropriated for the camparison of availabilities of the transmission units to precision spin- dles.