In order to find out optimum seed production date according to different defoliation and flooding period of Italian ryegrass, this studies with Tetrone were canied out on the experimental field of Honam Crop Experiment Station from 1986 to 1988. Treatments included cutting date of Nov. 20 and Apr. 10 and flooding period of 5-25 days. In soil after experiment, organic matter, phosphate and silicate content increased, but potassium content decreased 0.16% than that before experiment. Heading and maturing date of Nov. 20 cutting were same with those of non cutting, those of Apr. 10 cutting lates 6 days to heading date and 2 days to maturing date. Plant height and culm length of Nov. 20 cutting were sirniller to those of non cutting, those of Apr. 10 cutting were shorter and panicle length have no difference between non cutting and cutting. Lodging of cutting treatment was reduced than that of non cutting. Lodgin was increased as flooding period was long, also loding of all treatment occured at 30 days after heading. Two cutting times of Nov. 20 and Apr. 10 have the most fresh yield, while non cutting have the most dry matter yield. Optimun seed productin date was considered to suitable when 35 days after heading (Jun. 14), at this time, seed production was 1,640 to 2,640 kg/ha. Also if flooding j u r y have, seed production was good between 10 days and 15 days after flooding.