유충호르몬 유사물(juvenile hormone analogue: JHA)이 곤충의 난모세포 성숙에 미치는 영향을 확인하기 위하여 methoprene(isopropyl(2E, 4E)-11-methoxy-3, 7, 11-trimethyl-2, 4- dodecadienoate)을 꿀벌부채명나방(Galleria mellonella)의 번데기에 도포처리(topical treatment)하여 우화한 성충의 난모세포에서 유리 아미노산의 조성과 함량을 HPLC로 조사하였다. Galleria mellonella 난모세포 내의 유리아미노산 구성은 대조군과 methoprene 처리군에서 alanine을 비롯하여 총 16종을 동정하였다. 아미노산 함량은 대조군과 methoprene 처리군 모두에서 glutamic acid가 가장 높게 나타났으며, 대조군의 경우 alanine, proline, threonine, serine 등이 비교적 많은 함량을 보인 반면, asparagine, isoleucine, phenylalanine 등은 낮은 함량을 나타냈다. methoprene 처리군에서는 lysine, proline, arginine, alanine 등이 높게 나타났으며 asparagine, isoleucine, valine 등의 함량은 낮았다. 총함량도 전체적으로 methoprene 처리군이 대조군 보다 감소하여(P<0.01), methoprene이 난모세포 성숙 과정에서 유리아미노산 함량에 억제 효과를 나타내었다.
The effects of methoprene, juvenile hormone analogue. on free amino acid compositions of oocytes in the wax moth, Galleria mellonella were analysed by means of HPLC. A total of 16 free amino acids were found. Glutamic acid(control:1.33pmol/mg, methoprene treatments:0.44pmol/mg) were predominant amino acids in both groups. In control groups, the level of alanine(1.26pmol/mg), proline(1.24pmol/mg), threonine(0.70pmol/mg) and serine(0.66pmol/mg) were high. Also asparagine, isoleucine and phenylalanine were lower amounts. The contents of lysine(0.38pmol/mg), proline(0.33pmol/mg), arginine(0.27pmol/mg) and alanine(0.24pmol/mg) were relatively abundant, and those of asparagine, isoleucine and valine were low amounts in methoprene treatment groups. For significance level 1%(p<0.01), there exist differences between control and methoprene treatment groups. Therefore, free amino acid contents of the methoprene treatment groups were lower than those of the control groups. So we found that methoprene had a effects on free amino acid compositions of oocytes in the methoprene treatment groups.
