In order to find out the effect of temperature and shading on the growth and major disease infection of Eutrema wasabi Matsum., experiments were at growth chamber condition and field. Plant height, petiole and rhizome of wasabi were obvious decreased with increase the temperature, from which the optimum air temperature for the plant growth were estimated to be 17$^{circ}C$. The light intensity of the treatment with 75% shading net was favorable to the length of main rhizome, rhizome weight and whole plant weight. Infection ratio of black leg and soft rot were increased at high temperature condition, however their infection ratio were decreased with increase the shading condition.