This study was carried to find out relationships between water content and sugar content in fruit and vegetables and present the possibility of estimating sugar content by measuring water content. Water content and sugar content of melons, peaches, and apples were measured. Both F-test and linear regression analysis on the experimental data were done. The regression models were developed and validated. The results were summarized as follow : 1. According to the F-test, the coefficients of determinant were greater than 0.8, and significance probability was less than 0.0001 in each materials. 2. According to the regression analysis of melons, peaches, and apples, the standard errors of calibration(SEC) were 0.67 brix%, 0.82 brix%, and 0.72 brix%, respectively. 3. The standard errors of prediction(SEP) were 0.76 brix%, 1.03 brix%, and 0.77 brix%, respectively. 4. The relationship between sugar content and water content in apple was successfully applied to measure the sugar content from hydrogen NMR spectrum of apple samples.