This study was conducted to determine the inheritance of duvatrienediol(DVTs) and cis-abienol concentration from flesh tobacco leaves. NC744, which has a very low levels of DVTs and no cis-abienol was used as a parent in cross to TI 1068 producing a normal amount of DVTs and cis-abienol. Presence of DVTs and cis-abienol on the leaf surface was determined using thin layer chromatography(TLC). Segregation pattern from F$_2$ and BC$_2$[ (TI 1068 $ imes$ NC744) $ imes$ NC744J generations revealed that TI 1068 have a single dominant gene controlling DVTs and cis-abienol production. And DVTs production was inherited independently of the ability to produce cis-abienol.