The purpose of this study is to define requisite content of physiology for nursing education. This study classifies the subjects of physiology into 15 areas. The areas are cell and cell membrane, body fluid, nervous system, special sense, muscular system, blood, cardiovascular system, respiratory system, urinary system, digestive system, energy metabolism, body temperature, immune system, endocrine system, and reproductive system. Each subject area is further classified into subarea, resulting in a total of 194 subarea. The importance of each subarea is measured with a 3-point scale using a questionnaire. The subjects of this study were full-time professors teaching nursing in Korean universities. The analysis of the data collected from 68 respondents is as follows. 1. The areas of physiology necessary for nursing education in the order of importance are : body fluid, blood, endocrine, immune system, body temperature, urinary system, respiratory system, digestive system, reproductive system, energy metabolism, nervous system, cardiovascular system, cell and cell membrane, muscular system, and special sense. 2. Depending on the specific areas of nursing(such as pediatric nursing, maternity nursing), the importance of each physiology area may differ. For instance, the most important area for maternity nursing is reproductive physiology, whereas one for the psychiatric nursing is neuro-physiology. 3. The importance of each physiology area does not determine the importance of its subarea. For example, while the importance of cell and cell membrane was relatively very low across the respondents from different areas of nursing science, the importance of such subarea as osmosis, diffusion, and filteration reported by most respondents were high.