The present study was carried out to investigate the effect of Ni on germination, cell elongation, ${alpha}-amylase$ activity, contents of chlorophyll and protein in radish were determined in the water culture. As the concentration of Ni was increased in the water culture, germination of radish was 55% by Ni 10 mg/kg and 30% by Ni 20 mg/kg. The ratio of cell elongation injury was 50%, by two days after Ni 20 mg/kg treatment. The injury ratio of ${alpha}-amylase$ activity was 45% in the same condition and as the time goes on, inhibition of ${alpha}-amylase$ activity were slightly decreased. Contents of chlorophyll a and b were decreased two days after treatment and chlorophyll a was more inhibited than chlorophyll b. Also changes of the protein contents was slightly decreased. Activity of ${alpha}-amylase$ was decreased at germination stage, contents of chlorophyll a and b were decreased at growing stage.