To estimate the variation of food organisms on the corrugated PVC sheets, we analysed the chlorophyll a and. pheopigments of the benthic microalgae on the sheets from the oyster culture ponds. We estimated the standing crops as the amounts of chlorophyll a, and the physiological state of cells as the pheopigments level in the benthic microalgae. The amount of chlorophyll a was increased by immersion time in winter but the peak was observed every two weeks in summer, while the ratio of pheopigments was highest in summer but the low level was observed in spring and autumn. These seasonal variation were well coincided with the natural spawning season and the good microalgae nutritional valance.