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인삼 근부병균 Cylindrocarpon destructans (Zinssm.) Scholten의 포자 생성에 미치는 배양기간, 온도, pH의 영향
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  • 인삼 근부병균 Cylindrocarpon destructans (Zinssm.) Scholten의 포자 생성에 미치는 배양기간, 온도, pH의 영향
저자명
조대휘,유연현,오승환,이호자
간행물명
고려인삼학회지
권/호정보
1996년|20권 1호|pp.88-95 (8 pages)
발행정보
고려인삼학회
파일정보
정기간행물|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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The effects of media, incubation time, temperature and pH on production of conidia and chlamydospore of Cylindrocarpon destructans (Zinssm.) Scholen causing root rot of Panax ginseng were studied. Microconidia of the pathogen were abundantly produced on V-8 juice agar as a solid substrate with 5.73(log conidia/mm2) and in V-8 broth as a liquid substrate with 6.65 (log conidia/ml) among media tested. No difference was observed on the length of microconidia produced from the media with a range of 9.50∼11.38 $mu extrm{m}$. However, tryptic soy agar produced the broadest microconidia (average 5.00 $mu extrm{m}$) among the media tested. All the media produced chlamydospores In a range of 1.06∼4.37 (log chlamydospores/mm2) without a significant difference in number, while V-8 juice agar produced the bigger one (18.39 $mu extrm{m}$ in diameter) as compared to the tested media. The fungus began to sporulate conidia after three days of incubation and reached maximum at the 8th day. It seemed to be in a stationary phase until 30 days of incubation but was decreased thereafter. Chlamydospore was produced at 4th day after incubation. Maximum production was observed at 8th day and the number seemed to be maintained during the observation period. Both conidia and chlamydospore of the pathogen were able to be spoluated at 10∼25$^{circ}C$. However, optimum temperatures of conidia and chlamydospore formation were 15∼25$^{circ}C$ and 10∼20$^{circ}C$, respectively. C. destrmtans produced conida with an wide range of pH from 3.3 to 8.0 and chlamydospore from 2.8 to 8.0. Number of conidia was increased with an increase of pH up to 4.0. There was no significant difference in the number between 4.0 to 8.0. It seemed to have two optimum pH ranges, 3.3∼4.0 and 7.1∼8.0 for the chlamydospore formation.