A strain producing $alpha$-1, 3-glucanase was isolated from soil samples. The strain has a grey aerial mycelium and showed brown color from the other side. The temperature and pH range for growth were from 10$circ$C to 42$circ$C and from 4 to 10, respectively. Arabinose, dextrin and mannose were utilized for growth. Analysis of cell wall components revealed that the strain was classified as type I. From the results, the strain was identified as Streptomyces sp. By using this strain, the maximum production of 0.65 units/ml for $alpha$-1, 3-glucanase was achieved at 37$circ$C for 48 hrs.