We have studied the magnetoresistance and the magnetic anisotropy of Cu/(NiFe/Ni/NiFe) metallic multi layers grown on Si(100), Si(111), $4^{circ};tilt-cut;Si(111)$ or glass substrate. When the multilayer was grown on $4^{circ};tilt-cut;Si(111)$ with $50;{AA}$ of Cu underlayer, an in-plane uniaxial anisotropy was observed. On the other substrates such as Si(100), Si(111) or glass with Cu underlayer, however, no appreciable anisotropy was shown. The multilayer grown with NiFe or Ni underlayer or without underlayer did not show any arnsotropy even on $4^{circ};tilt-cut;Si(111)$. When $10;{AA}$ of NiFe was deposited prior to the Cu underlayer, the anisotropy in Cu/(NiFe/Ni/NiFe) multilayer disappeared.