In the sequential MPI system with one injection for each cycle, engine performance is influenced by the mixture conditions. It can be said that engine performance is improved by being better identical mixture formation conditions for all cylinders. As the fuel injection timing to the intake port effects on the mixture formation conditions and the engine performance, injection timing must be better adjusted to engine requirements. Engine behavior was clearly different depending on the injection time during intake storke. Therefore it was studied that injection timing of fuel effects on the engine performance I. e. combustion stability, COV(imep), A/F excursion, CO,HC emission concentration and fuel consumption. It was found that late intake-synchronous injection was deteriorated the combustion characteristics and performance characteristics, while early intake-synchronous infection resulted in favorable engine behavior.