배후발생과정 동안에 꿀벌부채명 나방의 뇌에 분포하는 leucokinin I 면역반응성 뉴런의 변화양상을 관찰하였다. 유충의 뇌의 경우, 모든 leucohinin I 면역반응성 뉴런들은 전뇌에만 좌우대칭으로, 중앙과 측면부위에 존재하였다. 또한 이들의 축삭은 망을 형성하면서 좌우대칭으로 아래로 뻗어 나갔다. 용의 시기가 되었을 때는 leucokinin I 면역반응성 뉴런의 수가 급격히 감소하여 성충시기까지 그 수가 유지되었다.
The changes of neurons and its distribution, using an antiserum raised against a neuropeptide, leucokinin I (LK I), were studied in the brain of the first, third, fifth, seventh instar larva and prepupa, 0-day-old, 5-day-old pupa and adult from the wax moth Galleria mellonella. All LK I-Immunoreactive neurons were found in the protocerebrum In the brain of the third instar larva, about 12-14 LK I-immunoreactive (LK I-IR) neurons were found. Of these LK I-IR neurons, four cell bodies were intensely detected in the median region and the remainders seen in the lateral region. In case of the fifth instar larva, total 12 LK I-IR neurons are distributed. When it became to the 7th instar larva, the number of LK I-IR neurons was increased and the median neurosecretory cell group (MNSC) and lateral neurosecretory cell group (LNSC), dorsolaterally in the protocerebrum were clustered in each hemisphere. Especially, the axons of each LK I-IR cells located in the median and lateral lesion were bilaterally descended and formed with meshwork. During the metamorphic changes from prepupa to adult, the number of LK I-IR neurons was decreased and remained with two.
