Several major agronomic traits of 4 isogenic lines for tillering(Kindred, Morex)were investigated under the different seeding rates and seedling mortality grown in the pots. The normal-tillering phenotypes had higher grain yield(46％) and leaf area index(56％) than their uniculm isogenic counterparts. The assortment rate(69％) and 1,000 grain weight(27％) in uniculm type were greater than the normal type. Uniculm plants were earlier in heading(4∼8days) and maturing(2∼3 days) dates compared with their counterparts. The grain yield was fitted to linear regression, ranged 0.659g to 0.5g per spike number. On increasing the seedling mortality rates, the grains per spike and 1,000 grain weight were slightly decreased, but the grain yield per plot was decreased markedly because of decrease of spikes per plot. The assortment rate, leaf photosynthetic capacity and reproductive tiller number were increased with increase of seedling mortality. The grain yield was largely dependent on the number of spikes per plot on occurrence of seedling mortality. For the uniculm genotypes to be introduced and bred into a present leading variety, the isogenic lines were precisely tested in the open field for a couple of years.