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Molecular Characterization of the Region Encoding Integrative Functions from Enterococcal Bacteriophage ${phi}$FC1
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  • Molecular Characterization of the Region Encoding Integrative Functions from Enterococcal Bacteriophage ${phi}$FC1
저자명
Kim. Min-Jung,Lee. Jin-Young,Kim. Young-Woo,Sung. Ha-Chin,Chang. Hyo-Ihl
간행물명
Journal of biochemistry and molecular biology
권/호정보
1996년|29권 5호|pp.448-454 (7 pages)
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생화학분자생물학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Bacteriophage ${phi}FC1$ is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage ${phi}FC1$ integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.