This Study was carried out to investigate the secondary cental nuclei innervating rat parotid gland. PRV-BaBlu as a neuronal tracer was injected into the left parotid gland and brains obtained through cardiac perfusion were treated by immunohistochemical staining. The results were as follows: L. The secondary central nuclei innervating rat parotid gland were paraventricular nucleus and central part of amygdaloid complex largely in diencephalon. 2. The paraventricular nucleus and central part of amygdaloid complex in diencephalon showed morphological asymmetry between PRV-BaBlu injected site and uninjected one. 3. The Ratio between total neurons and PRV-BaBlu infected neurons in paraventricular nucleus was $27.62{pm}16.23\%$ in left and $12.78{pm}8.69\%$ in right. 4. The Ratio between total neurons and PRV-BaBlu infected neurons in central part of amygdaloid nucleus was $14.25{pm}9.26\%$ in left and $8.35{pm}6.26\%$ in right.