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Effect of Humidity on Somatic Embryogenesis in Cotyledon Explant Culture of Carrot
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  • Effect of Humidity on Somatic Embryogenesis in Cotyledon Explant Culture of Carrot
  • Effect of Humidity on Somatic Embryogenesis in Cotyledon Explant Culture of Carrot
저자명
Lee. Eun Kyong,Cho. Duck Yee,Soh. Woong Young
간행물명
Journal of plant biology
권/호정보
1997년|40권 2호|pp.89-94 (6 pages)
발행정보
한국식물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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In order to clarify the influence of low humidity culture on the structure and function of somatic embryo the cotyledon explants of Daucus carrota L. cv. Hongshim wre cultured in the petridish whose lids had holes sealed with millipore filters. In the low humidity culture, the production of somatic embryos was enhanced and their maturation promoted but the cotyledon structure of somatic embryos were nearly similar to control. In addition, the low humidity culture improved the germination of somatic embryos. Especially, the germination frequency of jar-shaped embryos was much improved (68%) in comparison with that of jar-shaped embryos formed in constant humidity culture (23%). But low humidity culture at its extreme became an obstacle to normal plant regeneration in that precocious embryos were generated and the primary embryos turned into callus and formed secondary embryos. Therefore it is suggested that moderately low humidity culture (80-90% R.H.) is important to the higher production and better-quality of somatic embryos.