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Effect of Ammonium lon on Morphogenesis from Cultured Cotyledon Explants of Panax ginseng
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  • Effect of Ammonium lon on Morphogenesis from Cultured Cotyledon Explants of Panax ginseng
  • Effect of Ammonium lon on Morphogenesis from Cultured Cotyledon Explants of Panax ginseng
저자명
Choi. Yong Eul,Soh. Woong Young
간행물명
Journal of plant biology
권/호정보
1997년|40권 1호|pp.21-26 (6 pages)
발행정보
한국식물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Cotyledon explants of Panax ginseng were cultured on modified Murashige and Skoog medium with various concentrations of NH4Cl and KNO3. Morphogenesis such as somatic embryo, embryogenic callus, or adventitious root formation from cotyledon explants differently occurred according to the concentrations of NH4+ and NO3-. Somatic embryos were actively formed in a moderate concentration of NH4+(20 mM) in combination of NO3-, but in a high concentration of NH4+ (60 mM), only embryogenic calli were formed. In little or no NH4+, adventitious roots were formed at a high rate. the influence of NO3- on those morphogenesis was slight but combination of NO3- with NH4+ was indispensable since the cotyledon explants were necrotized on medium containing only NH4+ as a nitrogen source. Histological observation revealed that somatic embryo and embryogenic callus formation occurred from the same origin (cotyledon epidermis), whereas, adventitious roots were originated from the cells near vascular strands.