An experiment was conducted to figure out the atomization characteristics of a highly viscous biodiesel fuel with rice-barn oil applying and ultrasonic energy into it. A spray simulator for the droplet atomization, an ultrasonic system, and six different nozzles(3 pintle-type nozzles and 3 single hole-type nozzles) were made. To investigate effects of ultrasonic energy in a highly viscous liquid fuel, an immersion liquid method was used as a measurement method on droplet size distributions. It was found that the ultrasonic energy was effective for the atomization improvement of the rice-bran oil as a highly viscous biodiesel fuel and the factor나 such as the nozzle opening pressure, pin-edge angles, hole diameters, and collection distances affected the atomization of spray droplets.