Effects of fuel injection timing on the lean misfire limit of a sequential MPI SI engine has been investigated. To investigate the interaction of injection timing and intake flow characteristics, so called axial stratification phenomena, 4 kinds of different intake swirl port of the same combustion chamber geometry have been teated in a single cylinder engine test bench. And 2 kinds of fuel, gasoline and compressed natural gas(CNG), were used to see the effect of liquid fuel vaporization. Result shows that combination of port swirl and injection timing governs the lean misfire limit and lean misfire limit envelopes remain almost the same for a given ratio regardless of engine speed. It is also found that two phase flow has some effects on lean misfire limit.