For the experimental measurement of heat flux of DI diesel engine combustion chamber, the instantaneous temperature probes and data acquisition system were developed. By the analysis of measured temperatures at the cylinder head, the temperature at the point 3 which is located between intake and exhaust valve was higher than that of the other points. Temperatures at the point located mear the exhaust valve were higher than those of intake valve. The instantaneous and mean temperature at the cylinder head increases proportionally to the increase of the engine speed, while the temperature swing varies inversely. Temperature swings have influence on the maximum heat flux values from gas into head surface. It has been verified that these probes and data acquisition system perform well by the comparison of the trend of instantaneous temperature variation with that of measured combustion chamber pressure variation with respect to crank angle. It is presumed that these probes could be used in the measurement of other parts of combustion chamber as piston, cylinder wall etc. for the future study.