Biological control of turfgrass insect pest Blitopertha orientalis, forest insect pests, Agelastica coerulea, Meganola melancholia, and Glyphodes perspectalis,vegetable insect pests, Plutella xylostella and Agrotis segetum were conducted with entonopathogenic fungus, Metarhizium anisopliae J-22 isolated from black pine forest soil in Cheju province. Mortality of B. orientalis larvae was 53.3% at the rate of 3.4 $ imes$ 1O 7 conidia /ml. A. coerulea and M melancholia larvae showed 100% mortalities at 9.6 $ imes$ 106 conidia /ml and 2.7 $ imes$ 10 7 conidia /ml as well. However, G. perspectalis larvae were not dead even at 4$ imes$ 1O 7 conidia /ml. On the other hand, M anisopliae J-22 was effective against P. xylostella larvae showing 100% mortality at 4 $ imes$ 10 7 conidia /ml. KEy words:Entomopathogenic fungi, Biological control, Metarhizium anisopliae, Pathogenicity,Blitopertha orientalis, Agelastica coerulea, Meganola melancholia, Glyphodes perspectalis,Plutella xylostella, Agrotis segetum.