The cloging phenomenon in the fixed film reactor Is shown when biomass growth Is excessive for long operating time. In addition, effluent water Quality gets worse because of detachment of biomass. In this study, we conducted air-backwashing to sustain biomass In reactor to complement these defects. The results of experimental are showed In the following conclusion. The detachment rate was 19.5 - 38.0% when the organic loading rate was 0.40 - 1.32 kg COD/<TEx>$m^3$</TEX>/day, the k - backwashing Intensity was 2 L/min(6.7 $m^3$/$m^2$/hrl and the backwashing time was 15 - 19 seconds. And the detachment rate was 32.2 - 58.6 % when the organic loading rate was 1.37 - 2.27 kg COD/$m^2$/day, the backwashing time was 1 - 12 minutes. As orgnic loading rate and backwashing time ale Increased, detachment of fixed biomass Is Increased. The detachment equation with detachment rate(DR, %), backwashing time(BWT, min), fixed biomass concentration(FB. mg/L), and organic leading rate(OLR, kg COD/ms/day) through multiple linear regession was given by the following equation: DR : 17.964 $BWT^{0.1407} FB^{0.0597} OLR^{0.1946}$