Effects of DIF on stem elongation in plug seedlings of mini tomato ‘Mini Carol’ and on the growth and flowering after transplanting were investigated. Stem elongation of seedlings was mainly influenced by day temperature rather than night temperature. When the effect of ＋DIF was compared to that of -DIF under the same average daily temperature(ADT), day temperature had greater impact on internode elongation than night temperature. On the other hand, leaf unfolding rate increased and compactness decreased as ADT in creased. Differences in internode length affected by DIF during seedlings stage DIF progressively diminished during growth period after transplanting. Node position at which first flower was initiated was lowered as ADT decreased. It was concluded that DIF treatment was an applicable tool for control of stem elongation, particularly to reduce stem length without a noticeably adverse effect on the growth and development of plant after transplanting.