Triacylglycerols of the seeds of Ginkgo biloba have been resolved by high-performace liquid chromatography(HPLC} in the silver-ion and reverse-phase modes. The fatty acids were identified by a combination of capillary gas chromatography and gas-chromatography /mass spectrometry as the methyl and /or picolinyl ester. The main components are $C_{18:2{omega}6}$(39.0mol%), $C_{18:1{omega}7}$(asclepic acid 21.5mol%), and $C_{18:1{omega}9}$(oleic acid, 13.8mol%). Considerable amounts of unusual acid such as $C_{20:3{Delta}^{5,11,14}$ (5.7mol%), $C_{18:2{Delta}^{5,9}$(2.8mol%), and $C_{18:3}{Delta}^{5,9,12}$(1.6mol%), were checked. In addition, an anteiso-branched fatty acid, 14-methylhexadecanoic acid, was also present as a minor component(0.9 mol%). The triacylglycerols were separated into 17 fractions by reverse-phase HPLC, and the fractionation was achieved according to the partition numnber(PN) in which a ${Delta}^5$-non methylene interrupted double bond($^5$-NMDB) showed different behaviour from a methylene interrupted double bond in a molecule with a given cahinlength. Silver-ion HPLC exhibited excellent resolution in which fractions(23 fractions) were resolved on the basis of the number and configuration of double bonds. In this instance, the strength of interaction of a ${Delta}^5$-NMDB system with silver ions seemed to be weaker than a methylene interrupted double bond system. The principal triacylglycerol species are as follows ; $(C_{18:2{omega}6)2}/C_{18:1{omega}7}$, $C_{18:1{omega}9}/C_{18:1{omega}7}/C_{18:2{omega}6}$, $(C_{18:1{omega}7)2}/C_{18:2{omega}6}$, $C_{16:1{omega}7}/C_{18:1{omega}9}/C_{20:3}{Delta}^{5,11,14}$, $C_{16:1{omega}7}/C_{18:1{omega}7}/C_{20:3}{Delta}^{5,11,14}$, $C_{18:1{omega}9}/C_{18:1{omega}7}/C_{18:2{omega}6}$, $C_{18:1{omega}9}/C_{18:2}{Delta}^{5,5}/C_{20:3}{Delta}^{5,11,14}$, $(C_{18:1{omega}7)2}/C_{18:2{omega}6}$ and $(C_{18:1{omega}9)2}/C_{18:2{omega}6}$, while simple triacylglycerols without $C_{18:2{omega}6})_3$ were not present. Stereospecific analysis showed that fatty acids with ${Delta}^5$-NMDB system and saturated chains were predominantly located at the site of sn-3 carbon of glycerol backbones. It is evident that there is asymmetry in the distribution of fatty acids in the TG molecules of Ginkgo nut oils.