The purposes of this study were to determine wether knee muscular fatigue affects on one-leg static standing balance. Sixty four healthy subjects were used for this study : 44 men and 20 women, with an average age of 19.52. One leg static standing balance was measured at pre-fatigue and post-fatigue by an instrumented balance assessment system(kinesthetic ability training balance platform) which is commercially available for testing or training balance. Isokinetic exercises were used to evoke muscle fatigue at 180 degree/see by Cybex 1200. One leg static standing balance ability was significantly decreased after knee muscular fatigue. Although these phenomenons were not clearly understood, these results have important implications for rehabilitation in fatigable patients. These results suggest that the excessive fatiguing during rehabilitation in patients with fatigable disease may increase risk of reinjury and falling injury due to balance disturbance. Further studies are required to determine the physiological mechanisms of muscle fatigue that can play in decreasing one-leg static standing balance ability.