홍화 MeOH 추출물의 n-BuOH 분획으로부터 silica gel column chromatography를 반복하여 2종의 flavonoid 배당체를 분리, 정제하였다. 화합문의 화학구조를 HMBC를 포함한 NMR, MS, IR과 같은 기기분석 결과와 산가수분해반응을 이용하여 결정, 각각 $3-O-[{eta}-D-glucopyranosyl(1{ ightarrow}2);{eta}-D-glucopyranosyl];kaempferol$ 및 $3-O-[{alpha}-L-rhamnopyranosyl(1{ ightarrow}6);{eta}-D-glucopyranosyl];kaempferol$로 동정하였다. 각 화합물은 Grb2-Shc 결합저해활성$(IC_{50}:43,;47;{mu}g/ml)$을 나타내었다.
The MeOH extracts obtained from the flower petals of Carthamus tinctorius were solvent-fractionated with EtOAc, n-BuOH, and $H_2O$, successively. From the n-BuOH extract 2 flavonoid compounds were isolated through the repeated silica gel column chromatographies. From not only the results of physico-chemical data including HMBC but also the adaptation of acid hydrolysis, the chemical structures of the compounds were determined as $3-O-[{eta}-D-glucopyranosyl(1{ ightarrow}2);{eta}-D-glucopyranosyl];kaempferol$ and $3-O-[{alpha}-L-rhamnopyranosyl(1{ ightarrow}6);{eta}-D-glucopyranosyl];kaempferol$. The compounds exhibited $IC_{50}$ values in Grab2-Shc activity to be 43 and $47{mu}g/ml$, respectively.
