This study was to determine whether iontophoresis application of centipede venom would produce analgesic effect for clinical practice. Sixty patients suffered from musculoskeletal diseases who lived in Taejon city were analyzed by double-blind central study. After pre treatment, post treatment, post 20 minutes, post 40 minutes iontophoresis using centipede venom, we assessed the response to pin-prick sensation with Endomed 582 in myalgia patients. The results were as follows, control group showed pain threshold of 1.76, 2.03, 2.01, 2.02mA after treatment, but the pain threshold of 1.76, 2.21, 3.85, 3.87mA after iontophoresis application in study group. The pain threshold of Notermans pain score after 20-minute and 40-minute centipede venom iontophoresis group using centipede venom was higher than that after non-centipede venom iontophoresis group. The results showed that by the increasing the analgesic effect in the centipede venom iontophoresis group, especially in time of post 20 minutes after iontophoresis treatment. So we considered that the iontophoresis using centipede venom could be reduced pain of myalgia in musuloskeletal disease patients.