This research was investigated which denitrification of wastewater containing nitrate, using upflow anaerobic sludge blanket process. The upflow anaerobic sludge blanket process is also used for both artifical and industrial wastewater. Main ingredients investigated in the artifical and industrial wastewater experiment were the determination of optimum organism/nitrate ratios, nitrate removal efficiency by various hydrogen donor addition and characteristics of granular sludge and gas production in case of various hydrogen donor addition. From the experimental results the following conclusions were made: In case of adding methanol, ethanol and sodium acetate as hydrogen donor granular sludge was formed 50 days after seeding. Average diameter of granular sludge was 4.0 mm and settling velocity was 37 cm/min. Production rate of gas 3.3 L/d in case of adding methanol as hydrogen donor in wastewater containing 150mg/L nitrate. However adding ethanol and sodium acetate as hydrogen donor, gas production rate were 2.2-2.7L/d respectively. In case of adding methanol as hydrogen donor treatability of artifical wastewater contained 150mg/L as nitrate was about 93%. But in addition of sodium acetate in wastewater contained 40mg.L as nitrate, nitrate removal efficiency was 80%.